On-line coupling of gradient-HPLC and H-1 NMR for the analysis of random poly(styrene)-co-(ethyl acrylate)s

摘要

Full Paper: Random poly(styrene)-co-(ethyl acrylate)s can effectively analysed with respect to the chemical composition distribution by on-line coupled HPLC/H-1 NMR. The separation of the copolymers is conducted on a reversed-phase column using acetonitrile/tetrahydroturan as the eluent mixture. Via on-line coupling, the chromatographic peaks are directly transferred into the NMR spectrometer and analysed on-flow. Information on the chemical composition and the chemical heterogeneity of copolymers with high conversion is obtained. The experiments have been carried out using conventional HPLC-grade solvents and no deuterium lock. The results of the on-line HPLC/H-1 NMR investigation have been correlated with a HPLC procedure based on calibration with narrow-distributed copolymer standards. References: 14