AbstractTwo fish and seven invertebrate freshwater species were exposed to diflubenzuron (Dimilin®) in acute and chronic laboratory tests. No effects on newly hatched and juvenile fathead minnows or juvenile guppies were seen at or below 36 μg/L, the highest concentration tested. An early‐life‐stage test (30‐d) with fathead minnows showed no effect at or below 36 μg/L. No effects on survival, growth or reproduction were observed with two snail species,Juga pliciferaandPhysaspp., at or below 36 μg/L. Adult emergence of the caddis flyClistoronia magnificawas inhibited at 0.1 μg/L.Daphnia magnawere killed at 2.0 μg/L.Hyalella aztecamortality was significant at 2.0 μg/L. Molting and survival of the midgeTanytarsus dissimiliswere affected at 4.9 μg/L. Molting and survival of the midgeCricotopusspp. were affected at 4.9 μg/L, and adult emergence did not occ
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