Release of Histamine from Skin duringiin vitro/iAnaphylaxis

摘要

An in vitro system for the study of type I anaphylactic reactions in skin is described. Thin skin slices from sensitised rats are incubated with specific antigen and release of histamine is measured. Some simple characteristics of this system are defined. In further experiments histamine release is shown to be temperature-dependent and inhibition of release by disodium cromoglycate (Intal) is demonstrated.