Synthesis, characterization, and antioxidant activity study of a novel Pt-cyclopropa60fullerene complex/graphene oxide nanozyme

摘要

Over the past few decades, multifunctional hybrid bio-nanomaterials have emerged as nanozyme antioxidants, which are a new interdisciplinary frontier between nanotechnology and life sciences. In this study, we have successfully synthesized a novel Pt-cyclopropa60fullerene complex (C-60/Pt-I-CI) incorporating a-keto stabilized phosphorus ylide in a three-component reaction of the unsymmetrical phosphorus ylides Ph2P(CH2)PPh2 =C(H)C(O)C6H4-m-Cl, C-60 and Pt-2(dba)(3) (dba = dibenzylideneacetone). In the next step, by immobilizing this complex on graphene oxide (GO) nanosheets, the novel GO-Pt nanozyme (C-60/Pt-I@GO) was synthesized. Finally, cytotoxicity of the developed C-60/Pt-I@GO was examined by MTT assay against NIH3T3 cells. According to the results, this nanocomposite showed suitable cytocompatibility even at a relatively high concentration of the sample (25 mu g mL(-1)) in both 24 and 48 h. In detail, the viability of the cells for the highest concentration was obtained as 93.7 and 90.2 after 24 and 48 h, respectively. The alpha,alpha-diphenyl-1-picrylhydrazyl (DPPH) assay was applied to evaluate the antioxidant activity of C-60/Pt-I@GO nanocomposite. The received result not only did signify the antioxidant activity of this nanocomposite but also revealed a clear dose-dependent antioxidant activity for the produced nanocomposite. Also, the result of the time-dependent evaluation of C-60/Pt-I@GO nanocomposite antioxidant activity could confirm that they could reduce DPPH overtime to reach almost a plateau after 6 h of treatment, which could reveal the persistence of C-60 /Pt-I@GO nanocomposite to function effectively over time. Overall, C-60/Pt-I@GO nanocomposite was successfully produced and found effectively functional in reducing the DPPH radical, so they could be considered as a promising candidate to be studied as a novel nanozyme antioxidant.