Indexing of banana cultivar Karpura ChakkarakeU (AAB) for Banana Bract Mosaic Virus (BBrMV) by DAC-ELISA technique

摘要

Viral pathogens are major impediment in the banana tissue-culture industry. Among the viral diseases, BBrMV (Banana bract mosaic virus,), CMV (Cucumber mosaic virus,), BSV (Banana streak virus) andBBTV (Banana bunchy top virus,) are occurring severelyand causing major losses to banana farmers. Among the different native banana cultivars in Andhra Pradesh, Karpura ChakkarakeU (AAB) is one of the popular banana cultivar and occupied in an area of35,000 ha. Unfortunately, this cultivar is highly succumbed to Banana bract mosaic virus (BBrMV), genus Potyvirus, the causal agent o/bract mosaic disease, is considered to be the most threatening and causing great yield loss. In the light of severe spreading of the viral diseases during recent times there isan utmost need to increase the production supply of disease free planting material as per the demand which is raising day by day from the potential banana growing districts in the state Andhra Pradesh. The area expansion has, however, been accompaniedby rampant spread of pests and diseases, most important among which are viral diseases transmitted through non-indexedplanting material. Thus, the needfor production ofdisease free, quality planting material is being felt more than ever before not onlyin the state but in other regions as well. Early detection by means of sensitive diagnostic methods is the main way to control them. Direct Antigen Coating Enzyme Linked Immuno Sorbent Assay (DAC-ELISA) a serological method utilized in the present was found effective in the detection of BBrMV infected mother plants of banana cv. Karpura ChakkarakeU (AAB).