EVALUATION OF ANTIFUNGAL ACTIVITIES OF NEWLY SYNTHESIZED BIMETALLIC CYANIDO COMPLEXES UNDER LABORATORY CONDITIONS

摘要

The aim of the present study was to search the in vitro antifungal activity of the bimetallic cyanido complexes coded, K1 (Ni(hydeten)2Ag(CN)2Ag(CN)2.H20, K2 Cd2(hydeten) 2Ag4(CN)8.H20, K3 Ni(bishydeten)2Ag(CN)2Ag(CN)2.H20, K4 Cu(bishydeten)2Ag2 (CN)4, K5 Cd(bishydeten)0.52Ag(CN)24.3H20, K6 Ni(N-bishydeten)Ag3(CN)5, K7 Cu(Nbishydeten)Ag3(CN)5, K8 Zn(N-bishydeten)Ag3(CN)5, K9Cd(N-bishydeten)4Ag(CN)28Ag(CN)2, K11 Cu(edbea)Ag2(CN)4.H20, K12 Cd(edbea)2 Ag(CN)22.H20 and K13 Cd(edbea)Ag3(CN)5 against plant pathogenic fungi, Alternaria solani, Rhizoctonia solani and Fusarium oxysporum f. sp. lycopersici. Inhibitory effects of the compounds were evaluated by well diffusion method with different concentrations (5, 10, 15, 20, 25, 30, 35 ve 40 pg/ml). Beside the positive control Maneb (2.4 mg/ml) and cupper hydroxide (1.25 mg/ml), negative control 50 dimethyl sulfoxide was used. Results demonstrated that most of the compounds inhibited the mycelial growth of the fungi. K13 was highly effective and suppressive the radial mycelial growth of F. oxysporum f.sp. lycopersici, while K9 exhibited maximum inhibition (98) on mycelial growth of R. solani at 40 pg/ml. However, K4 was found to be the less effective in suppessing F. oxysporum f.sp. lycopersici. It was also observed that radial mycelial growth of selected pathogens reduced with increasing concentration of test compound. LC50 values of the compounds against myce- lia growth of tested fungi changed between 6 to 24.5 pg/ml. LC90 values changed 19 to 198.7 pg/ml. 0f the tested compounds K1 the most effective one with 90.51 mycelial growth inhibition at 20 pg/ml. All the tested compounds except K4, K7 and K11 resulted in 88.79 to 100 growth inhibition at 30 and 40pg/ml. K4, K7 and K11 were not effected the mycelial growth of the pathogen even at 40pg/ml. Similar effect was observed in spor germination test. The compound K11 did not inhibited the spor germination of the pathogen at all concentrations tested. The present study reports that the bimetallic cyanido complexes could be used as an alternative in control of the phytopathogenic fungi tested.