Ultrasound-assisted extraction of bioactive compounds from giloy (Tinospora cordifolia) stem: Quantitative process optimization and bioactives analysis

摘要

Giloy (Tinospora cordifolia), a medicinal herb, contains several bioactives having immunomodulatoryproperties and various health benefits. In this study, giloy stem extractwas derived using ultrasound process followed by screening of secondary metabolitesand bioactives. Box–Behnken design coupled with RSM was employed for the optimizationof yield, total phenolic content and antioxidant activity of GSE with respect tosolvent-to-solid (SS) ratio, sonication time (ST), and ultrasound amplitude (UA). The UA,ST and their interaction effects had significantly influenced the green extract yield andquality. The optimum extractions conditions were found to be 30 (UA), 10:1(SS ratio), and 30 min (ST). The optimum responses were obtained as follows: extractyield: 9.614 ± 0.015, TPC: 100.04 ± 0.133 (mg GAE/100 g extract), and DPPH inhibition: 78.8523 ± 0.399. Screening of optimized extract through HR-LCMSQTOFreported the presence of major polyphenols such as malic acid, catechin, andquercitrin with a match factor of 99.23, 98.65, and 90.58, respectively.