Prevalence of Transferable OXA-1 (3-Lactamase Associated with Carbapenem-Resistant Klebsiella pneumoniae Isolates in Iraq

摘要

This study was designed to explore the incidence of bla_(QXA1) amongst Klebsiella pneumoniae isolates with resistant to carbapenem. Between December 2014 and April 2015, one hundred samples were taken from two hospitals: Babylon Teaching Hospital forMaternity and Pediatric / Babylon Province (clinical, umbilical infections, n= 40; environmental, n=20) and Karbala Hospital for Pediatric / Karbala Province (40 stool samples). All patients were hospitalized or attended these hospitals, all under 1 yearof age. Seventeenth (17) isolates were identified as Klebsiella pneumoniae. The antibiotic resistance profile of isolates was tested using disk diffusion method. High-level of resistance was recorded with ampicillin (94.1) and piperacillin (88.2) antibiotics. Resistance to carbapenem was reported in two K.pneumoniae isolates, these were investigated for the existence of OXA-lactamase using Polymerase Chain Reaction (PCR) technique. Two (100) isolates gave positive result. Transference of this genewas studied by conjugation experiment. The bla_(OXA-1) gene conjugated successfully in 1 (50) isolate only.