Identification of the Pink Pigment from Pinkish Barley Grains and a Simple Discrimination Method to Detect Pink Seed Diseasi

摘要

In recent years, pinkish grains of barley (Hordeurn vulgare L.) have occasionally been observed in different areas of Japan, and are perceived to have lower quality. Here, we extracted a pink pigment from pinkish barley grains and identified it as proferrorosamine A, based on cation exchange resin fractionation and UPLC-QTOF-MSE analysis. Furthermore, a pink pigment extracted from bacteria isolated from pinkish grains and cultured on potato dextrose agar (PDA) was similarly identified as proferrorosamine A. These results indicate that the pinkish barley grains observed in Japan are infected with "pink seed disease." We describe the use of a simple pink seed disease discrimination method, using 48-well PDA plates containing FeS04 and antifungal agents, to detect infection in whole grains, pearled grains, rice-shaped barley and hull-less and hulled barley.