Evaluating biohydrogen production by Clostridium hydrogenum sp. nov. strain CUEA01 isolated from mangrove sediments in Thailand

摘要

The hydrogen (H-2) fermentative Clostridium hydrogenum sp. nov. strain CUEA01 was isolated from a mangrove sediment in Thailand. Genome sequencing and analysis revealed a genome size of 5,501,482 bp that encoded for 3,292 predicted protein coding genes with annotated functional assignments and many genes associated with carbon utilization and H-2 evolution. The H-2 production performance was evaluated in batch fermentation, and revealed that this strain can grow and produce H-2 at a broad range of temperatures (15-40 degrees C), pH (4-10), and initial glucose concentrations (5-60 g/L). The maximum H2 yield (3.11 molH(2)/molglucose) was obtained at 37 degrees C, pH 8, and an initial glucose concentration of 10 g/L. Furthermore, this strain could utilize various carbon sources, including xylose, xylan, starch, mannose, glycerol, and avicel cellulose, amongst others. Additionally, CUEA01 was compatible with agro-industrial wastes and could achieve a maximum CHP of 4639 mL/L and 4024 mL/L from sugarcane molasses and cassava pulp, respectively. This demonstrates that CUEA01 has a potential for H-2 fermentation from complex organic wastes since it can secrete enzyme cocktails that consolidate the fermentation process. (c) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.