Characteristics of an Orange-Pigmented Bacterium Isolated from a Hot Spring in Kagoshima, Japan

摘要

In May 2012, strain HNN-6 (=JCM 18566), a Gram-negative, non-spore-forming, motile and strictly aerobic rod, which produces a pale orange pigment, was isolated from a hot spring water sample obtained in Kagoshima, Japan, by a plating method using R2Amedium at 30°C for 7 d. The 16S rRNA gene sequences (1,437bp) of this strain (accession number: AB731137) had a close similarity (99.1%) to Hydrotalea sandarakina AF-51~T (JF739858). Growth occurred at 25-45°C and pH 5.0-8.0, with optimal growth at 40°C and pH 6.0-7.0. Growth did not occur in the presence of >=2% NaCI. The API 20NE identification system gave positive results for nitrate, aescuiin, gelatin, 4-nitrophenyl-beta-D-galactopyranoside, D-glucose, D-mannose, maltose and oxidase (API code number 1472204). The dominant cellular fatty acids of strain HNN-6 were iso-C_(16:0) (32.6%), iso-C_(17:0) 3-OH (24.2%) and iso-C_(16:0) (8.4%). The guanine-plus-cytosine (G+C) content of DNA was 36.2 mol%. This article is the first report to describe the characteristics of an orange-pigmented bacterium isolated from a hot spring water sample in Japan.