A New Simple Method for Determination of Partition Coefficient by Normal Phase Thin Layer Chromatography (TLC).

摘要

Owing to me importance of partition coefficient in field of pharmaceutical education and research in the present work, We have made and attempt to determine benzene/water partition coefficient (logP) values for drugs by simple normal phase TLC technique. The partition coefficient being the relative concentration of drug in benzene/water, remains as an important physiochemical parameter which determine the absorption of drug in the body and thus influence it therapeutical response. According to literature the determination of partition coefficient is done by shake-flask method, GC, HPLC and by potentiometric method. These methods are accurate but expensive. Here there is need for simple and efficient method to determine logP value for desire drug compound or pharmaceutical research. This reasserted to come out with simple experiment technique like normal phase TLC to determine to calculate logP value. And which can be the part of under graduate laboratory practical. The differential values like Rfb/w and logRfb/logRfw were calculated and found to be closer to reported log P values (i.e from chemical abstract ACD lab), Paracetamol-0.461, Naproxen-0.769, Atenolol-0.22, Hydrochlorthiazide-0.331, Telmesertan-5.11 Pthalic acid-0.85, Sulfathiazoline-0.1, Nimesulide-0.9, Diclofenac-0.2, Benzoic acid-0,9. Considering the results we have concluded that simple and convenient TLC method was developed to determine logP values, which were matching with reported logP values of drugs bank.