Comparative Biochemical Changes in Resistant and Susceptible Cotton Cultivars to Leaf Curl Virus at Germination and Early Seedling Stage: a-amylase, Starch, Total Soluble Sugars in Seed, Radicle and Plumule

摘要

Biochemical changes in resistant and susceptible cotton cultivars to leaf curl virus (CLCuV) were assessed by a series of laboratory experiments. The experiments were conducted in petri-dishes containing filter papers with sterile distilled water on w hich seeds of the cotton cultivars i.e.. CIM-446, resistant and S-12 susceptible to CLCuV were sown, each cultivar have five replications and petri-dishes were kept in growth cabinet at 28± 2°C. The results showed that the growth attributes proved S-12 superior to CIM-446. But with respect to the biochemical attributes both the cultivars appear to use different biochemical attributes for their germination demands. Soluble sugars translocations and its concentrations were higher in CIM-446 than S-12, I n all the three embryonic organs of germinating seeds, starch mobilization had also the same pattern of varietal difference, where CIM-446 had greater ratio of starch, more active enzyme (alphan-amylase), degrading starch and higher comparative ratio of the resultant product of starch degradation. This may provide better fulfillment of structural requirements to resist virus at vegetative growth stages by contributing readily available energy by active break down of starch and translocation of sugars wh ich may play some role in the composition of antibodies or some other biochemical/physiological responses associated with starch and sugars.