Calli induction through anther culture in peach-tomato plants (Solanum sessiliflorum Dunal).

摘要

Flower buds from peach tomato plants (Solanum sessiliflorum) were collected to the establishment of a protocol of calli induction from anthers culture. Aseptic cultures were achieved after the immersion of explants into 70% ethanol for 45 sec followed by 0.8% sodium hypochlorite for 5 min. Cultivated anthers developed 50 and 70% of calli with 0.1 mg L-1 indole-3-acetic acid (IAA)+5 mg L-1 kinetin (KIN) and 0.1 mg L-1 indole-3-acetic acid (IAA)+10 mg L-1 kinetin (KIN), respectively. The calli oxidation increased in treatments free of activated charcoal, daily pre-refrigeration (4 degrees C for 48 h) and 20 mg L-1 of kinetin (KIN). The best plant growth regulator concentration for stimulating calli formation was 5 mg L-1 of kinetin (KIN).