Identification and Pathogenicity of Fusarium Species Associated with Root Rot and Stem Rot of Dendrobium

摘要

Three Fusarium species, F. oxysporum, F. proliferatum and F. solani were isolated from root and stem rot of Dendrobium orchid. Their pathogenicity on Dendrobium was studied and the inoculation test showed that the three Fusarium species were pathogenic, causing root and stem rot on the orchid. Molecular characterization using PCR-RFLP of ITS+5.8S regions showed that the isolates from the same species produced similar patterns and UPGMA cluster analysis of PCR-RFLP of ITS+5.8S clearly grouped F. oxysporum, F. proliferatum and F. solani into separate clusters. The present study showed that three Fusarium species, F oxysporum, F. proliferatum and F. solani were associated with root and stem rot of Dendrobium orchid.