Evaluation of hydrogen fermentation by a newly isolated alkaline tolerant Clostridium felsineum strain CUEA03

摘要

A new alkaline tolerant Clostridium felsineum strain (CUEA03) was isolated from a mangrove sediment in Thailand. Genomics revealed that this strain contained a 5,081,113 bp genome sequence with 4797 predicted protein coding sequences, comprised of a large number of genes that are linked to several processes of carbon utilization, hydrogen (H2), and butanol production. Chemical tests revealed that the bacteria have a high potential for utilization of a wide range of carbon sources. After optimization by batch fermentation, a maximum cumulative H2 production (CHP) of 5425 mL/L in 72 h was obtained at pH 9, 30 degrees C, 0.9 g/L NaCl, and 35 g/L glucose. Moreover, this strain was compatible with agro-industrial waste, giving a CHP of 5187 mL/L (893 mL/g CODconsumed). This demonstrates that environmentally isolated organisms have the potential to be used as robust high H2 producers from various complex organic substrates.(c) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.