HPLC Separation of Phenolic Acids on Silica Gels Layer-by-Layer Modified with 6,10-Ionene and Dextran Sulfate

摘要

Using phenolic acids as an example,we continue to study the nature of the previously obtained multilayer sorbents for HPLC-silica gels modified up to two times with 6,10-ionene and dextran sulfate(DS).The chromatographic behavior of this class of compounds on the obtained sorbents was studied.A six-component mixture of sorbic,vanillic,sinapic,p-coumaric,caffeic,and ferulic acids was separated on the silica gel twice modified with 6,10-ionene and dextran sulfate in 15 min with a selectivity of up to 1.88 and a column efficiency of up to 26000 theoretical plates per meter.The possibility of separating the two classes of compounds-phenolic acids and basic nitrogen-containing pharmaceuticals-in a single mixture on silica gel,twice modified with 6,10-ionene and dextran sulfate,is shown.