In vitro evaluation of metabolites of Trichoderma spp. against second stage juveniles of Meloidogyne incognita.

摘要

The metabolites of T. harzianum and T. viride were extracted with acetone, benzene, methanol, and ethyl acetate using soxhlet extractor. The different concentrations (1000, 500, 250 and 125 ppm) of these extracts were evaluated against J2 [second-stage juveniles] of M. incognita in vitro by immersion bioassay method. Nematode mortality was recorded after 24, 48 and 72 h of exposure. Based on mortality data, LC50 values were calculated by probit analysis. The LC50 values of these extracts were compared with the LC50 value of the control (triazophos). The ethyl acetate extract of both fungi were the most effective. The extracts of T. harzianum was superior to those of T. viride irrespective of the solvent used. Methanol was moderately efficient, whereas acetone and benzene were the least efficient.