A Novel Coumarin-Based Fluorescent Probe for Sequential Detection of Al~(3+)and H2PO_4~-

摘要

A coumarin-derived acylhydrazone probe(L)was designed and synthesized for successive detection of Al~(3+)and H2PO_4~-in DMSO/H2O solution.The probe L exhibited high selectivity towards Al~(3+)with visible color change and prominent fluorescence enhancement at 592 nm,while the in situ prepared L-Al~(3+)complex could selectively recognize of H2PO_4~-via fluorescence quenching.The detection limits for Al~(3+)and H2PO_4~-were calculated to be 3.4 and 11.0 nM,respectively.The binding ratio of L to Al~(3+)was obtained as 2:1 according to the Job’s plot and ESI-MS while the association constant was determined to be 2.13×10~4 M~(-1).Furthermore,the probe L was successfully applied in detection of Al~(3+)and H2PO_4~-with test strips and in real water samples.