首页> 外文期刊>Autonomic neuroscience: basic & clinical >Three types of putative presympathetic neurons in the rostral ventrolateral medulla studied with rat brainstem-spinal cord preparation.
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Three types of putative presympathetic neurons in the rostral ventrolateral medulla studied with rat brainstem-spinal cord preparation.

机译:用大鼠脑干-脊髓制备研究延髓腹侧延髓中三种类型的假定性交感神经元。

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To study the electrophysiological properties of presympathetic neurons in the rostral ventrolateral medulla (RVLM), intracellular recordings were performed by the whole-cell patch-clamp technique. We utilized the neonatal rat brainstem-spinal cord preparation, in which the sympathetic neuronal network is thought to be preserved, unlike in slice preparation. In response to stimulation in the ipsilateral Th2 spinal segment including intermediolateral cell column (IML), 33 of 151 non-respiratory RVLM neurons showed antidromic action potentials with a constant latency of 45 ms, and can be considered as presympathetic neurons. We classified and characterized the RVLM presympathetic neurons into three types: 'regularly firing neurons (n=7)', which showed ramp depolarization and frequent action potentials (4.2+/-0.9 spikes/s) with rare excitatory postsynaptic potentials (EPSPs); 'irregularly firing neurons (n=21)', which exhibited many EPSPs that modulated the firing rate; and 'silent-type neurons (n=5)', which discharged action potentials only during current-induced depolarization. Lucifer-Yellow staining showed that the irregularly firing neurons were significantly larger and had more dendrites than the regularly firing neurons. All regularly firing neurons retained their discharges during low-Ca2+ -high-Mg2+ superfusion that blocks synaptic input, whereas the discharges in 11 of 16 irregularly firing neurons were abolished, suggesting that the regularly firing neurons discharged independently of synaptic input. Seven of 31 RVLM neurons were hyperpolarized by stimulation of vagal afferent nerves. In summary, three types of RVLM presympathetic neurons were characterized by the patch-clamp technique in the brainstem-spinal cord preparation, in which the connection was preserved from vagal afferent to the Th2 spinal segment through the RVLM. Since antidromic action potentials were demonstrated by stimulation in the Th2 spinal segment in 33 neurons of all three types, all types of RVLM neurons constitute a part of the sympathetic neuronal network.
机译:为了研究前臂腹外侧延髓(RVLM)中交感神经元的电生理特性,通过全细胞膜片钳技术进行了细胞内记录。我们利用新生大鼠脑干-脊髓制备,其中与切片制备不同,认为交感神经元网络得以保留。响应同侧Th2脊柱节段(包括中间外侧细胞柱(IML))的刺激,在151个非呼吸性RVLM神经元中,有33个表现出抗皮肤动作电位,并具有45 ms的恒定潜伏期,可以被认为是交感神经元。我们将RVLM交感神经元分为三种类型:“正常放电神经元(n = 7)”,表现出斜坡去极化和频繁的动作电位(4.2 +/- 0.9尖峰/秒),具有罕见的兴奋性突触后电位(EPSPs); “不规则发射神经元(n = 21)”,表现出许多调节发射速率的EPSP;和“沉默型神经元(n = 5)”,仅在电流引起的去极化过程中才释放动作电位。 Lucifer-Yellow染色显示,不规则发射神经元比规则发射神经元大得多,树突更多。在低Ca2 +-高Mg2 +融合过程中,所有规则激发的神经元都保持放电,从而阻止突触输入,而16个不规则激发神经元中的11个放电被取消,这表明规则激发神经元的放电与突触输入无关。 31个RVLM神经元中有7个因迷走神经的传入而超极化。总之,在脑干-脊髓制备中,膜片钳技术表征了三种类型的RVLM交感神经元,其中通过RVLM保留了从迷走神经传入到Th2脊髓节段的连接。由于通过刺激在所有三种类型的33个神经元的Th2脊髓节段中显示了抗驱动作电位,因此所有类型的RVLM神经元均构成交感神经元网络的一部分。

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