Fabrication of an Electrochemical Sensor Based on Silver Nanoparticles Stabilized by Phoenix dactylifera Seed Oils for the Determination of Ornidazole in Milk Samples

摘要

In this study, silver nanoparticles (Ag-NPs) were synthesized using AgNO3 through a simple route using bio-oil obtained from flash pyrolysis of Phoenix dactylifera seed powder as reducing as well as capping agent. The acquired bio-oil was characterized by FT-IR and GC-mass spectrometry. Besides, the main objective of this study is to construct a highly sensitive sensor to detect ornidazole (ODZ) in real samples. The sensor was elaborated by using carbon paste electrode modified with the synthesized Ag-NPs (Ag-NPs /CPE). The synthesized Ag-NPs were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and UV-Visible (UV-vis). The resulting sensor demonstrates a significant shift in ODZ reduction peak toward more positive potential (-0.257 V .vs Ag/AgCl) compared to the unmodified CPE (-0.561 V .vs Ag/AgCl). The ODZ peak reduction current Ipc varies linearly with ODZ concentration in the range of 8.0x10(-6) to 1.0x10(-3) mol L-1 with a detection limit of 9.76x10(-7) mol L-1. The proposed method has been successfully used to determine ODZ in milk sample with satisfactory results.