We describe a set of dental and postcranial horse material recovered from the locality Los Hornitos, northeastern Sonora, northwestern Mexico. The fossil-bearing unit consists of brown silt with intercalation of fine-grained sandstone and bioturbated incipient soils, set in an alluvial environment and considered Pliocene in age. The comparative study of the available sample indicates the presence of two species: the small-sized hipparionine Nannippus peninsulatus and the medium-sized and primitive equine Equus simplicidens. The isotope composition of 813C and 818O in dental enamel tooth indicates a C3-C4 mixed diet for these species and suggests the presence of woodland and open areas in Los Hornitos during the Pliocene. The less negative 813C values observed in E. simplicidens are related to a more C4 diet, preferring to roam open habitats. The coexistence and optimal resource partitioning between these species are explained, considering their differences in dietary behavior, body size, home range, and/or vagility. The recognized horse species are typical of the Blancan North American Land Mammal Age, and their biochronological information from the southwestern United States and central Mexico establishes the probable age of the Los Hornitos at about 3.9-2.6 Ma, corresponding to the Blancan III or late early Blancan - early late Blancan.
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