Fluorescent Gramicidin Derivatives for Single-moelcule Fluorescence and Ion Channel Measurements

摘要

Single-molecule spectroscopiesin combination with single-channel patch-clamp measuements have thepotential for providing new information on ion channelgating processes.Fluorescent gramicidin derivatives is known and themechanism of gating (peptide dimerization) is generally agreed.We describe here the synthesis and characterization of two pairs of gramicidin derivatives that should prove useful for such studies.They contain robust fluorophores,undergo resonance energy transfer(FRET) when they dimerize,and have single-channel properties close to those of the wild-type channel.