The aim of the study was to evaluate callus induction and phenolic compound production of Digitalis purpurea L.(foxglove) callus extracts derived from leaves and formed in MS medium supplemented with different NaCl concentrations.NaCl decreased callus induction and callus fresh weight at all concentrations.The highest total phenolic compound contents were recorded from callus grown in mediums with 75 and 100 mM NaCl supplements,compared with the callus induced in the medium containing only MS.Among the six phe-nolics found in the callus samples,catechin,caffeic acid and apigenin were the major constituents.The amount of catechin increased significantly in callus grown in 100 mM NaCl medium by nearly 10-fold and the amount of caffeic acid and apigenine increased significantly in callus grown in 125 mM NaCl medium by nearly 7 and 20-folds (respectively) compared with the control group.Major volatiles in foxglove calli were represented by cro-tonaldehyde,3-methylbutanal,2-methylbutanal,ethanol,furfural,n-hexane,limonene and dimethyl sulfide.
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机译：本研究的目的是评估洋地黄（毛地黄）愈伤组织提取物的愈伤组织诱导和酚类化合物的产生，这些愈伤组织提取物来源于叶片，并在添加不同NaCl浓度的MS培养基中形成。NaCl 降低了所有浓度下的愈伤组织诱导和愈伤组织鲜重。与在仅含有MS的培养基中诱导的愈伤组织相比，在添加75和100 mM NaCl的培养基中生长的愈伤组织记录了最高的总酚类化合物含量。在愈伤组织样品中发现的6种苯酚酸类物质中，儿茶素、咖啡酸和芹菜素是主要成分。与对照组相比，在100 mM NaCl培养基中生长的愈伤组织中儿茶素的量显着增加了近10倍，在125 mM NaCl培养基中生长的愈伤组织中咖啡酸和芹菜素的量分别显着增加了近7倍和20倍。毛地黄愈伤组织的主要挥发物以烯甲醛、3-甲基丁醛、2-甲基丁醛、乙醇、糠醛、正己烷、柠檬烯和二甲基硫醚为代表。
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