The effect of various concentration of Bovine Serum Albumin in Mice (Mus musculus) In vitro Embrio culture on the number of Blastocyst

摘要

This study aimed to determine the optimal concentration of Bovine Serum Albumin in increasing the amount of blastocyst. Oocytes were obtained from female mice Balb/C strains. Oocyte cells were collected and divided into 3 groups: T1 (MEM + BSA 3%), T2 (MEM + BSA 5%), and T3 (MEM + BSA 7%). Semen is collected from fertile male mice, then motile spermatozoa were put into a medium containing eggs. Media contained spermatozoa and eggs were stored in a 5% CO_2 incubator. Observations were made under an inverted microscope at 100x magnification. Embryos that developed to the blastocyst stage show with a clear cytoplasm, clear plasma membrane, no granularity, and clear pellucide zone.Based on statistical data analysis, there was a significant difference between the highest and lowest concentrations of p<0.05, with significant differences in the treatment group T1 (54.00~a ± 8.94) and the treatment group T3 (32.00~b ± 10.95). It can be concluded that the optimal concentration of Bovine Serum Albumin in supporting embryonic development is a concentration of 3%. The addition of Bovine Serum Albumin to the culture media was able to support the development of mouse embryos to the blastocyst stage.