A Mn-doped calcium phosphate nanoparticle-based multifunctional nanocarrier for targeted drug delivery and cellular MR imaging

摘要

Multifunctional nanoplatform integrated with efficient imaging for diagnosis and therapeutic functions are much promising for realizing highly efficient imaging-guided chemotherapy in vivo but still in a great challenge. The nanocarriers with biodegradable and good biocompatibility are an urgent requirement. Herein, we designed Mn-doped calcium phosphate nanoparticles (Mn-CaPNPs) for targeted delivery of doxorubicin (DOX) and MR imaging-guided cancer therapy. Mn-CaPNPs were synthesized via a double reverse emulsion approach. To obtain excellent colloidal stability, mPEG-NH2 was conjugated to Mn-CaPNPs by electrostatic adsorption. Doxorubicin hydrochloride (DOX) and EGFR Mab C225 were conjugated to the Mn-CaPNPs-mPEG-NH2 via hydrophobic interaction and the cross-linking reaction of glutaraldehyde (Mn-CaPNPs-DOX, Mn-CaPNPs-DOX-Mab C225). The as-prepared multifunctional nanocarrier nanoplatform possess good biocompatibility, high loading capacity for DOX, good T1-MR contrast effect, and significantly enhancing the targeted therapeutic effect against BxPC-3 cells. The results suggested that Mn-CaPNPs could be used as a promising nanocarrier nanoplatform for highly efficient imaging-guided cancer therapy.