Thin-Layer Chromatographic Separation of14C-Labeled Metabolites from Photosynthate

摘要

A method for separating14C-labeled metabolites (including key intermediates of the glycolate pathway) from plant extracts by thin-layer chromatography is reported. The method provides for rapid and convenient determination of the14C-content of metabolites in leaf samples obtained during kinetic investigations of photosynthesis with14CO2.