In this work, a novel chitosan Schiff base 4-(2-Hydroxyaniline)pent-3-en-2-one chitosan (2-HyA-CS) and its ZnO nanocomposite (2-HyA-CS/ZnO) were sensitized and characterized by appropriate methods; FTIR, XRD, Elemental analysis, SEM, TEM and TGA. The result of characterization methods confirms the preparation of 2-HyA-CS and 2-HyA-CS/ZnO. The SEM images reveal that chitosan, 2-HyA-CS, and 2-HyA-CS/ZnO have a varied roughness and porous surfaces. The reason for this difference was attributed to the formation of Schiff base 2-HyA-CS and the presence of ZnO nanoparticles in 2-HyA-CS/ZnO. The patterns of XRD and FTIR confirm the formation of 2-HyA-CS and 2-HyA-CS/ZnO. The degree of substitution (DS) of modified chitosan 2-HyA-CS was calculated using Elemental analysis and FTIR.ATR, it was found to be 74. The adsorption efficiency of the produced adsorbents was compared with pure chitosan to remove of Remazol Brilliant Blue R (RBBR) from an aqueous medium and antimicrobial activity. The removal percentage of RBBR by chitosan, 2-HyA-CS, and 2-HyA-CS/ZnO are 47.12, 91.9, and 96.56, respectively with the following order: 2-HyA-CS/ZnO > 2-HyA-CS > chitosan. Their antimicrobial activities were studied against two Gram negative bacteria (E. coli and P. aeruginosa), two Gram positive bacteria (S. aureus and B. cereus) and (C. albicans) as a yeast strain, the inhibitory zone measurements revealed that the activity of 2-HyA-CS/ZnO is excellent and higher than 2-HyA-CS and pure chitosan. The cytotoxicity of the prepared compound 2-HyA-CS and 2-HyA-CS/ZnO along with pure chitosan was estimated against two human cancer cells MCF-7 cells and HepG-2 cells, the result indicates that 2-HyA-CS/ZnO having higher Inhibitory activity against both MCF-7 and HepG-2 cells with 53.5 ± 2.86 and 27.4 ± 1.23 μg/mL respectively and 2-HyA-CS possessing moderate Inhibitory activity against both MCF-7 and HepG-2 cancer cells with IC_(50) = 2I6.5 ±7.48 and 135.6 ± 6.49 pg/ml respectively.
展开▼
