Effects of lipopolysaccharide-loaded PLGA nanoparticles in mice model of asthma by sublingual immunotherapy

摘要

The aim of this study was to encapsulation LPS into PLGA nanoparticles and evaluation of effects by SLIT in asthmatic BALB/c mice. LPS-loaded PLGA NPs were prepared in water-in oil-in water method. Sensitized BALB/c mice to ovalbumin as allergen, LPS-loaded PLGA NPs in different low and high doses were administered sublingually. Serum and splenocytes level of some cytokines, also, BALF and lungs histological analysis was performed. SLIT by LPS-PLGA 200nm treatment showed significantly increased the cytokine. The study of BALF and histological examination indicates the reduction of cells and eosinophilic infiltration. The LPS-PLGA NPs could effectively shifted Th2 to Th1/Treg.