Lupinus latifolius is a perennial herbaceous and one of the most nitrogen-fixing legumes.The bacterial strain that amplified part of thel6S rRNA gene,DT02,was isolated from the root nodule of L.latifolius planted in Nezahat Gokyigit Botanical Garden,Istanbul,Turkey.The isolated bacterium is Grampositive,rod-shaped,acidic,facultatively anaerobic,and fast-growing.gDNA was isolated from the bacterium,and amplified gDNA of the expected size were sequenced and analyzed using bioinformatics tools.The identified partial 16S rRNA sequence was submitted to the NCBI database (accession number MN599098.1).Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain DT02 belonged to the genus Rhodococcus within the family Nocardiaceae and was closely related to Rhodococcus sp.strain 137A-21with a similarity value of 99.93 %.This bacterium can have plant-growth-promoting abilities.Additionally,the rhizome of L.latifolius was also harvested and analyzed for mineral element content by ICP-OES analysis.According to the measurement,the rhizome was rich in terms of Ca (2000 mg kg-1),Na (153 mg kg-1),Mg (2000 mg kg-1),and K (143 mg kg-1).
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