Remineralization potential and biocompatibility of titanium dioxide-doped phosphate glasses

摘要

This study reports the remineralizing potential and biocompability of titanium dioxide doped phosphate glass (TDPG). Glass paste was prepared with polyacrylic acid and applied as a thin coat on etched dentin for different periods. Normal and etched dentin were used as controls. Remineralization potential was tested using atomic force microscopy, scanning electron microscopy coupled with energy dispersive x-ray and Raman spectroscopy. The biocompability was tested using XTT assay and human dental pulp stem cells. After 5 days of paste application, some angular or plate-like crystals were observed occluding some dentinal tubules and precipitated within intertubular dentin. After 14 days, these crystals form a thin, uniform layer, consistent with P-vs-O-P, vasPO2 and vsPO peaks and has ions detected in the glass, covering the dentin surface. By 30 days, the thickness of this layer and intensity of detected peaks increased to completely obscure the underlying dentin apatite peak. By 77 days, additional glass peaks were identified. The glass extract showed comparable or significantly higher cell viability than positive control.Conclusion: TDPG paste could be a suitable material for remineralization of affected dentin.