PCR TECHNIQUE FOR DETECTION OF SOME VIRULENCE ASSOCIATED GENES IN LISTERIA MONOCYTOGENES ISOLATED FROM TABLE EGGS AND CLINICAL HUMAN SAMPLES

摘要

The aim of this study was to assess the extent of Listeria monocytogenes in table eggs and its causation of human spontaneous abortions in Sohag city, Egypt by isolation methods and PCR analysis for the presence of virulence-associated genes. The result revealed that out of 30 pooled egg samples, 4 (13.3%) L. monocytogenes were isolated from egg shells only, among which 100% and 50% were revealed to encode inlA and hlyA virulence genes respectively, while recovered 5 (4.2%) L monocytogenes strains from clinical human samples, among which each of inlA and hlyA virulence genes were present in 2 (40%) of the isolated strains. The results give a spotlight on the association between L. monocytogenes from food and some virulence genes with human abortionin Sohag city.