The altered HLA‐DQ expression in peripheral blood T cells of chronic hepatitis B patients characterizes the function of T cells

摘要

Abstract Objective This study aimed to clarify the expression of HLA‐DQ and granulysin in peripheral blood T‐cell subsets in patients with chronic hepatitis B virus (CHB) and to evaluate their significance in assisting CHB diagnosis and immune status assessment. Methods Peripheral blood from 34 CHB patients, 36 inactive HBsAg carriers and 33?healthy controls were collected, and HLA‐DQ and granulysin in a series of T‐cell subsets were analysed by flow cytometry. The ability to secrete IL‐10 and IFN‐γ and the functional T‐cell subsets were measured in Treg and CD4 cells expressing HLA‐DQ or not. Correlation analyses were further conducted between HLA‐DQ/granulysin‐related subsets and clinical indicators of HBV infection, and ROC curves were built to evaluate diagnosis efficiency of HLA‐DQ‐related subsets. Results HLA‐DQ+ percentages in circulating CD4?T cells were downregulated in CHB patients. The proportions of HLA‐DQ?+?Tfh in CHB were upregulated while HLA‐DQ+ percentages in Treg were decreased. In terms of function, the IFN‐γ secretion ability of CD4?+?T cells and IL‐10?secretion in Tregs were stronger in HLA‐DQ+ than HLA‐DQ‐ subsets. HLA‐DQ?+?CD4?+?T cells and HLA‐DQ?+?Treg were negatively correlated with HBV‐DNA, while HLA‐DQ?+?Tfh and Tfc cells were positively correlated with HBV‐DNA and ALT. HLA‐DQ?+?Treg/Tfh/Tfc could help to distinguish CHB from inactive HBsAg carriers. Conclusion HLA‐DQ on T cells can characterize the function of T‐cell subsets and analysis of HLA‐DQ can help to evaluate immune status and assist in diagnosis of CHB.