Nucleic Acid (Calf Thymus-DNA, Yeast tRNA) Binding and Cytotoxic Properties of a Dinuclear (Ru,Co) Metal Polypyridyl Complex

摘要

Based on [L2Ru{DPPZ(11-11')DPPZ}RuL2]~(4+) (where L= 1,10-phenanthroline or 2,2'-bipyridyl, DPPZ(11-11')DPPZ= 11,11'-bi(dipyrido-[3,2-a:2',3'-c]-phenazinyl)), a heterodinuclear (Ru,Co) metal polypyridyl complex [(phen)2Ru{DPPZ(11-11')DPPZ}Co(phen)2]~(5+) (phen= 1,10-phenanthroline) has been designed and synthesized. A comparative study on the interaction of the complex with calf thymus DNA and yeast tRNA was investigated by UV-visible spectroscopy, fluorescence spectroscopy and viscosity measurements, as well as equilibrium dialysis and circular dichro-ism. The antitumour activities of the complex were evaluated by the 3 -(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraazolium bromide method and Giemsa staining experiment. These results indicate that the configuration and structures of nucleic acids have significant effects on the binding behaviours of metal complexes. Furthermore, the complex shows different antitumour activities against selected tumour cell lines, and can cause cell apoptosis.