Quantitative inoculation with dry conidia of Botrytis cinerea

摘要

A method for the application of dry Botrytis cinerea conidia with an apparatus suitable for use on the laboratory bench was designed and constructed. The release of increasing quantities of dry conidia resulted in increasing spore densities on the settling chamber base. A significant (P < 0.0001) linear regression relationship between dose and density was fitted to the data (R-2 = 0.83). The precision of dry conidial application was also examined and coefficients of variation of 10% and 12% in spore density on the settling chamber base were obtained.