Cytochrome bcl Complex:Potential Breach to Improve the Activity of Phenazines on Xanthomonas

摘要

The effects of the natural pesticides,phenazines,were reported to be limited by some tolerant metabolism processes within Xanthomonas.Our previous studies suggested that the functional cytochrome bcl complex,the indispensable component of the respiration chain,might participate in tolerating phenazines in Xanthomonas.In this study,the cytochrome bcl mutants of Xanthomonas campestris pv.campestris(Xcc) and Xanthomonas oryzae pv.otyzae(Xoo),which exhibit different tolerance abilities to phenazines,were constructed,and the cytochrome bcl complex was proven to partake a critical and conserved role in tolerating phenazines in Xanthomonas.In addition,results of the cytochrome c mutants suggested the different functions of the various cytochrome c proteins in Xanthomonas and that the electron channeled by the cytochrome bc1 complex to cytochrome C4 is the key to reveal the tolerance mechanism.In conclusion,the study of the cytochrome bcl complex provides a potential strategy to improve the activity of phenazines against Xanthomonas.