A Reversible NIR-II Ratiometric Fluorescent Probe for Real-Time In Vivo ATP Detection

摘要

ATP plays a prominent role in shaping the evolution of immune cell responsesto injury, infection, and cancer. However, real-time monitoring of ATP levels invivo remains a challenge, due to the lack of reliable tools that work reversiblyin deep tissues. Herein, based on the F?rster resonance energy transfer(FRET) strategy, a reversible ratiometric second near-infrared window (NIR-II)molecular fluorescent probe (CX-RATP) is designed and synthesized. CX-RATPexhibits a significant fluorescence ratio change with the existence of 0–10 mMATP in vitro, which is within the range of physiological and pathological ATPconcentrations. Meanwhile, the recognition process can be reversed byapyrase. It can capture the increase and decrease in ATP levels in real timebefore and after the treatment of acute inflammation. Besides, CX-RATP isalso successfully applied to the hepatocellular carcinoma (HCC) model andachieves in situ imaging of tumor lesions. These results all provide solid proofthat CX-RATP is a powerful tool for in vivo real-time ATP detection.