This work investigated the effect of static magnetic field (SMF) on Bacillus atrophaeus endospore germination. Germination was triggered by L-alanine in 1.3-T SMF and characterized by ion release, Ca2+-dipicolinic acid release, and water influx. These events were monitored by electrical conductivity, Tb-DPA fluorescence, and optical density, respectively. Culturability of endospore germinated in SMF exposure was evaluated by CFU enumeration. Results indicated that 1.3-T SMF failed to significantly affect endospore germination and culturability, suggesting that the three aforementioned processes were not sensitive to SMF. (C) 2016 Wiley Periodicals, Inc.
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机译:本研究探讨了静态磁场(SMF)对萎缩芽孢杆菌内生孢子萌发的影响。1.3-T SMF中的L-丙氨酸触发萌发,其特征是离子释放、Ca2+-吡啶甲酸释放和水流入。这些事件分别通过电导率、Tb-DPA荧光和光密度进行监测。通过CFU计数评估SMF暴露中萌发的内生孢子的培养能力。结果表明,1.3-T SMF对内生孢子萌发和培养性影响不显著,表明上述3个过程对SMF不敏感。(c) 2016 年 Wiley Periodicals, Inc.
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