Metabolic Changes in The Snake Head Fish Channa punctatus Due to Latices of Euphorbia royleana

摘要

Laboratory evaluation was made to assess the short as well as long term effects of methanolic extract of Euphorbia royleana latex against nontarget freshwater fish Channa punctatus, which shares the freshwater habitat with target animals. Exposure ofsublethal doses (4.70 mg centre dot l; 9.41 mg centre dot l for 24 h and 3.67 mg centre dot l and 7.34 mg centre dot l for 96 h) of methanolic extract caused significant (P<0.05) time and dose dependent reduction in the levels of total protein nucleic acids (DNA and ENA) glycogen, pyruvate and significant (P<0.05) enhancement in the levels of total free amino acid, lactate and activity of enzyme protease in both liver and muscle tissues of freshwater fish C. punctatus. Withdrawal study also shows that there is a significant recovery in all the above biochemical parameters, in both tissues of fish after the 7th day of the withdrawal of treatment, which supports the view that the plant product is safe to be used as pesticides for control of freshwater target organisms as well as predatory and weed fishes from freshwater ponds.