Larval culture of Scylla serrata: maintenance of hygiene and concepts of experimental design

摘要

Significant progress has been achieved towards sustainable commercial production of mud crabs from hatcheries. However, the larval rearing protocol used has not been the most efficient. In the culture of mud crab larvae, the maintenance of hygiene and the control of pathogenic organisms are critical to survival and metamorphosis. The control of pathogens in larval culture should not be dependent on antibiotics, as resistant organisms win develop. Biological control of pathogens using microbially mature seawater or probiotics has potential for the development of successful larval culture. Worthwhile experimentation with larval cultures must embody good statistical design and execution. Replications from at least two hatchings are necessary to establish firm conclusions on treatment effects. Factorial experiments can be used to reduce experimental error and wastage of materials as well as to investigate treatment interactions.