Screening Natural Products. Bioassay-Directed Isolation of Active Components by Centrifugal Partition Chromatography

摘要

A Centrifugal Partition Chromatograph (CPC), Model LLN by Sanki, was used on routine basis as a primary tool for over 200 assay-directed fractionations in search of active principles from extracts of natural products. Various extracts were found active in several receptor and enzymatic assays incorporated into a high capacity screening system targeting discovery of new antiinflammatory, immunomodulating and antiviral agents. Centrifugal partition chromatography is well suited for performing the assay-directed fractionations, since like other countercurrent techniques it does not involve solid phase adsorbents and thus is inherently less destructive. The instrument was found reliable, simple to operate and applicable to the entire range of polarity of natural products. It can handle milligram as well as multigram quantities.