Assessment of Poultry Feed ContaminationLevel by Aflatoxin B1: Quantification by TwoChromatographic Analysis Methods

摘要

Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety.Ingestion of contaminated feed by poultry can lead to impaired health andzootechnical performances but also a human diet safety problem related toresidues presence in animal origin products. Aflatoxin B1 contamination ofpoultry feed samples marketed in Dakar city and in peri-urban areas (Gorom,Sangalkam) was studied. A total of 15 samples were collected from Dakar citymarkets as well as from poultry farms in Gorom and Sangalkam areas. Afla-toxin B1 quantification was performed by high performance liquid chroma-tography and thin-layer chromatography. HPLC results showed that all sam-ples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar.Only the finishing feed from Gorom had an aflatoxin B1 level above themaximum limit set by regulations. TLC is a suitable method for aflatoxinsdetection. However, it was associated with overestimation for aflatoxin B1quantification. Results suggest that poultry feed represents a real source ofhuman diet contamination. In addition, HPLC remains the most reliablequantification technique for quality control.