Evaluation of Microsatellite Diversity in Common Bean Parental Surveys

摘要

Microsatellite markers are highly polymorphic genetic markers that have proven useful for genetic mapping studies in plants. The utility of microsatellites derives from the fact that they detect length polymorphisms at genetic loci that have simple sequence repeats and as a result are highly variable. Microsatellites have been developed for common beans from both non-coding (genomic) and coding (gene-derived) sequences that contain simple sequence repeats (Yu et al., 1999, 2000; Gaitan-Solis et al.,2002 and Blair et al., 2003). The objective of this work was to characterize microsatellite diversity in three sets of common bean genotypes that are parents of mapping populations at CIAT. The parents included wild and cultivated germplasm from both the Mesoamerican and Andean gene-pools, while the microsatellites evaluated included gene-based and genomic derived markers as categorized by Blair et al. (2003).