BIOASSAYS TO DIAGNOSE SELECTED BEAN POTYVIRUSES

摘要

A hot and dry growing season revealed many virus symptoms in commercial snap bean fields in upstate New York in 2005. Dr. Rosario Provvidenti suspected three potyviruses, Bean Yellow Mosaic Virus (BYMV), Clover Yellow Vein Virus (CYVV) and WatermelonMosaic Virus (WMV) in field samples of 'Hystyle' snap bean in Geneva, New York. Unfortunately, common immunosorbent assays (ELISA) are not able to resolve specific potyviruses, and bioassays have not been reported to make distinctions between these three potyviruses. This report highlights the development of practical bioassays using specific indicator plants for diagnosing the potyviruses: BYMV, CYVV and WMV in the bean virus complex.