A NEW INOCULATION PROCEDURE TO EVALUATE ANGULAR LEAF SPOT DISEASE IN BEAN PLANTS (Phaseolus vulgaris L.) FOR BREEDING PURPOSES

摘要

Angular leaf spot incited by the fungus Phaeoisariopsis griseola (Sacc.) Ferraris is a severe disease in common bean (Phaseolus vulgaris L.). This disease has been considered one of the main problems responsible for the low yield of this crop in Brazil. Pyramiding resistance genes to overcome diseases caused by pathogens has been proposed as an strategy to obtain durable and wide spectrum resistance. However, pyramiding can be a difficult task when only conventional inoculation techniques are used to evaluate the plant symptoms (Faleiro et al., 2003). The objective of this study was to develop an alternative method for inoculation of P. griseola using excised bean leaves.