CONSTRUCTING A GUS-TAGGED INFECTIOUS CDNA CLONE OF BEAN COMMON MOSAIC VIRUS

摘要

An infectious cDNA clone of Bean common mosaic virus strain RUl was constructed, between the 35S promoter and NOS terminator, using overlapped fragments of the virus that were amplified with the primer pairs designed on the basis of previously postedsequence of RUl (AY863025). To circumvent the toxicity of viral genome to Escherichia coli an intron was integrated into the viral genome and the whole cassette was introduced into Agrobacterium tumefaciens. Finally viral cDNA was tagged with the GUS gene for feasible study of BCMV-host interaction.