ABSTRACTThe detection of cold‐tolerant plasmid‐bearing pathogenic strains of Yersinia enterocolitica depends on the expression of various plasmid‐associated phenotypic properties. In this study, low calcium Congo red agarose medium was used as a single multiple test medium to identify pathogenic serotypes of Y. enterocolitica or to indicate the pathogenic potential of individual strains. The assay is based on Congo red uptake, crystal violet binding, low calcium response and hydrophobicity. In general, the efficiency and reliability of this medium was found to be better than existing dye binding techniques for detecting virulent Y. enterocolitica st
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