FACTORS AFFECTING PRODUCTION OF CELLULOSE FROM gluconacetobacter xylinus

摘要

Some Gluconacetobacter strains produce cellulose called bacterial cellulose (BC). BC is expected to be a commodity material in various fields of industry. Cellulose production by Gluconacetobacter xylinus NCIM 2526 was affected by the type and concentration of carbon source, nitrogen source, pH and temperature. Among the carbon sources fructose, mannitol and inositol were found to be suitable for optimum levels of cellulose production. BC yield was maximum at 4% (w/v) of inositol (6.0 g/1). The organism was able to utilize a wide range of nitrogen sources such as peptone, casein hydrolyzate, beef extract, and malt extract for cellulose production. Also the effect of pH, temperature and supplementary effect of metal ions & solvents on BC production had been evaluated. pH of 6.5 and 30 deg C temperature were found to be suitable for BC production. Addition of metal ions like zinc, magnesium, calcium, and nickel at a level of lOOppm and solvents like methanol and ethanol were found to be stimulatory for BC production. Using G. xylinus NCIM 2526 in the modified HS medium, the amount of cellulose produced was almost double (6.6 g/1) as compared to that in standard HS medium.