Rapid Micro Isolation of Thymosin #x3B1;1from Thymosin Fraction 5 by Reversed-Phase High Performance Liquid Chromatography

摘要

We have developed a rapid, efficient, and reproducible method for the purification of thymosin α1(Tα1) from thymosin fraction 5 (TF5). This procedure can serve as a model for isolation of other biologically active peptides from TF5 in sufficient quantity for characterization. The purification procedure is based on the use of high-performance preparative/semi-preparative and analytical reversed-phase (C18Delta-Pak) chromatographic columns. The HPLC retention time, pI, RIA, SDS-PAGE, and amino acid composition analysis have shown that natural, purified Tα1is identical to synthetic α1.