BIODEGRADATION OF ACID BLUE 113: KINETIC ANALYSIS AND PLACKETT BURMAN SCREENING DESIGN FOR MEDIUMCOMPONENTS

摘要

Four different bacterial strains have been isolated from dye containing effluent and are used for the construction of an aerobic consortium CN-1 along with Pseudomonas aeruginosa CLRIBL22 obtained from CLRI. These bacteria were identified as Citrobacter freundii (2 strains), Moraxella osloensis and Pseudomonas aeruginosa using 16S rRNA analysis. The organisms constituting the bacterial consortium CN-1 have started to degrade AB 113 in their middle lag phase. They exhibited difference only in the ratein which they carry out the dye degradation. The consortium CN-1 showed gradual increase in the rate of degradation which started in the middle lag phase and reached a maximum by lOh following first order reaction with the rate constant, kj value being0.152 h1. With respect to growth, the consortium showed an extended log phase before it reached its stationery phase by 24h. Screening for medium components using Plackett Burman Screening Design indicated dye concentration having larger effect followedby temperature and ammonium nitrate concentration. It can be seen with low concentration of dye and low concentration of glucose, dye decolorization by the consortium was predominating, provided temperature and ammonium nitrate concentration were held high with pH maintained at 9.